Discovery of a Novel Long Noncoding RNA <i>Lx8-SINE B2</i> as a Marker of Pluripotency

<div>Dissection of lncRNA <i>Lx8-SINE B2</i> promoter region. (a) Schematic of the positions of TEs on according to mouse mm10 genome the promoter region of lncRNA <i>Lx8-SINE B2</i>. (b) Schematic of various length fragments of lncRNA <i>Lx8-SINE B2</i> promoter constructs. (c) Activities of various length fragments of lncRNA <i>Lx8-SINE B2</i> promoter constructs were determined by <i>luciferase</i> reporter gene assays in E14 ESCs. Biological-triplicate data (<svg height="8.55521pt" id="M28" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -8.34882 27.8368 8.55521" width="27.8368pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z"></path></g><g transform="matrix(.013,0,0,-0.013,10.158,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z"></path></g><g transform="matrix(.013,0,0,-0.013,21.421,0)"><path d="M285 378C315 398 338 416 353 432C373 451 384 474 384 503C384 579 325 635 236 635H235C182 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279 437 253 421C222 403 201 385 180 371V452C135 432 85 419 41 411V388C99 379 102 374 102 310V103C102 38 93 32 27 26V0H238V26C189 32 180 38 180 103V338C210 363 250 390 289 390C351 390 377 348 377 275V103C377 37 368 32 306 26V0H520V26C465 32 456 38 456 101V296C456 314 455 326 453 338C491 369 529 390 565 390C628 390 653 345 653 274V107C653 36 642 32 583 26V0H797Z"></path></g></svg>.</span> (d) Luciferase assay analysis of core promoter activity of lncRNA <i>Lx8-SINE B2</i> in ESCs and 3T3 cells. Biological-triplicate data (n =3 extracts) are presented as mean ± s.e.m.</div>

Stem Cells International

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Figure 3

Figure 3: Discovery of a Novel Long Noncoding RNA <i>Lx8-SINE B2</i> as a Marker of Pluripotency 

Stem Cells International

Discovery of a Novel Long Noncoding RNA Lx8-SINE B2 as a Marker of Pluripotency

Figure 3