Discovery of a Novel Long Noncoding RNA Lx8-SINE B2 as a Marker of Pluripotency

Figure 4

Oct4 and Sox2 drive the expression of lncRNA Lx8-SINE B2. (a–c) qPCR analysis of lncRNA Lx8-SINE B2 expression after depletion of core transcription factors Oct4 (a), Sox2 (b), and Nanog (c) in ESCs. The data are represented as from three biological replicates. ns, non-significant; ; according to two-sided Student’s -test. Biological-triplicate data ( dishes). (d–f) Luciferase assay analysis of core promoter activity of lncRNA Lx8-SINE B2 after depletion of core transcription factors Oct4 (d), Sox2 (e), and Nanog (f) in ESCs. Biological-triplicate data ( extracts) are presented as . (g) Binding profile of Sox2, Oct4, and Nanog on the promoter region of lncRNA Lx8-SINE B2 according to published data as described in methods. Input was included as a control.

(a)

(b)

(c)

(d)

(e)

(f)

(g)