The Impact of Various Culture Conditions on Human Mesenchymal Stromal Cells Metabolism

<div>(a) Analysis of selected cytokines in the medium supplemented with 10% iFBS and 7.5% PRSRGF after cultivation with MSC (<span class="nowrap"><svg height="9.65783pt" id="M17" style="vertical-align:-0.3499298pt" version="1.1" viewbox="-0.0498162 -9.3079 60.2586 9.65783" width="60.2586pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M135 536C164 536 186 560 186 587C186 617 164 639 136 639C109 639 85 617 85 587C85 560 109 536 135 536ZM252 0V26C188 32 181 38 181 106V451C138 433 90 420 39 412V388C99 379 102 374 102 312V106C102 38 95 32 32 26V0H252Z"></path></g><g transform="matrix(.013,0,0,-0.013,3.484,0)"><path d="M493 503C489 551 484 614 483 650H43V622C120 616 128 611 128 525V126C128 40 120 34 40 28V0H312V28C221 34 213 40 213 126V307H316C407 307 412 296 424 227H453V420H424C412 355 407 346 316 346H213V584C213 613 216 616 246 616H322C398 616 419 607 436 579C449 559 455 539 464 499L493 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(b) Determination of protein fractions in nondiluted iFBS, PR-SRGF, AB-HS, AB-HS (Sigma), and ABS (adult bovine serum) (without cell cultivation) by capillary ELFO analysis compared to the human reference standard (albumin, alpha-1-globulins, alpha-2-globulins, beta-1-globulins, beta-2-globulins, gamma-globulins). Light grey: out of the reference range; dark grey: extremely out of the reference range.</div>

Stem Cells International

fig5

Figure 5

Figure 5: The Impact of Various Culture Conditions on Human Mesenchymal Stromal Cells Metabolism 