Pharmacological Preconditioning Improves the Viability and Proangiogenic Paracrine Function of Hydrogel-Encapsulated Mesenchymal Stromal Cells

Figure 5

Celastrol preconditioning increases paracrine proangiogenic activity. (a) HUVEC growth when cocultured with hydrogel-encapsulated hMSC. Alpha MEM 0.2% FBS and EGM were used as negative and positive controls, respectively (, , ); versus the vehicle at 24 h; versus the vehicle at 48 h; versus alpha MEM 0.2% FBS at 48 h. (b) Effect of paracrine activity of hydrogel-encapsulated hMSC on wound closure; , , ; negative control: alpha MEM 0.2% FBS; positive control: EGM ( versus the vehicle group at each time point).

(a)

(b)