Nsd2 Represses Endogenous Retrovirus MERVL in Embryonic Stem Cells

<div>Nsd2-mediated H3K36me2 is correlated with Ssrp1 binding. (a) Genomic distribution profile of H3K36me2, H3K36me3, H3K4me3, and Ssrp1. Color scale represents the strength of Pearson’s correlation. (b) ChIP-seq signal density enrichment of Ssrp1, H3K4me3, H3K36me2, and H3K36me3 (<span class="nowrap"><svg height="8.68572pt" id="M1" style="vertical-align:-0.0498209pt" version="1.1" viewbox="-0.0498162 -8.6359 8.6074 8.68572" width="8.6074pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M640 650H413L406 622L438 617C475 611 483 605 463 571C422 503 346 400 296 333C264 403 215 529 199 575C189 604 191 614 228 618L262 622L271 650H30L23 622C90 615 97 609 119 553L212 321C222 295 223 285 218 259L193 127C176 39 167 33 82 28L76 0H350L356 28C266 36 257 40 275 127L303 266C308 290 314 302 328 322C396 418 454 493 498 546C551 610 565 616 631 622L640 650Z"></path></g></svg>-</span>axis) on gene body from TSS to TES (<span class="nowrap"><svg height="8.68572pt" id="M2" style="vertical-align:-0.0498209pt" version="1.1" viewbox="-0.0498162 -8.6359 10.0819 8.68572" width="10.0819pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M748 650H522L515 622L546 617C580 611 587 604 565 575C518 513 469 451 419 393C376 474 349 534 330 580C318 609 325 612 361 618L383 622L392 650H151L144 622C214 616 224 612 257 543L360 327C270 218 187 124 159 95C106 40 92 34 26 28L17 0H252L259 28L236 31C189 37 188 47 209 78C249 136 308 210 377 294L478 79C494 44 487 37 449 32L418 28L409 0H673L680 28C596 34 591 39 554 116L436 361C526 469 574 521 604 553C659 612 669 614 739 622L748 650Z"></path></g></svg>-</span>axis). H3K36me3 enrichment increased from TSS to TES. (c) Relative mRNA expression of <i>Nsd1</i>, <i>Nsd2</i>, and <i>Nsd3</i> in ESCs according to qPCR. (d) qPCR analysis of <i>Nsd1</i>, <i>Nsd2</i>, and <i>Nsd3</i> expression levels in WT ESC and <i>Ssrp1</i><sup>-/-</sup> ESC; data are shown as <span class="nowrap"><svg height="7.35473pt" id="M3" style="vertical-align:-0.3499303pt" version="1.1" viewbox="-0.0498162 -7.0048 72.5244 7.35473" width="72.5244pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M797 0V26C739 32 732 36 732 103V296C732 394 682 449 605 449C576 449 550 437 529 423C504 407 475 389 446 366C425 418 382 449 334 449C303 449 279 437 253 421C222 403 201 385 180 371V452C135 432 85 419 41 411V388C99 379 102 374 102 310V103C102 38 93 32 27 26V0H238V26C189 32 180 38 180 103V338C210 363 250 390 289 390C351 390 377 348 377 275V103C377 37 368 32 306 26V0H520V26C465 32 456 38 456 101V296C456 314 455 326 453 338C491 369 529 390 565 390C628 390 653 345 653 274V107C653 36 642 32 583 26V0H797Z"></path></g><g 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Stem Cells International

Nsd2 Represses Endogenous Retrovirus MERVL in Embryonic Stem Cells

Figure 1