Comparison of the Donor Age-Dependent and In Vitro Culture-Dependent Mesenchymal Stem Cell Aging in Rat Model

Figure 1

(a) Comparison of the cell surface expression CD29 and CD90 from short-term (passage 3) and long-term (passage 30) in vitro cultures of rats 6 mth old and 2 yrs. old; (b) morphology of ASCs from short term (3 passage) of 6 mth old donors and 2 yrs. old donors and of ASCs from long-term (30 passage) culture of 6 mth old donors and 2 yrs. old donors; (c) flow cytometry analysis (FSC/SSC) of the granulation of ASCs from 2 yrs. donors, 3 p. (passage) and 30 p. and 6 mth donors, 3 p. and 30 p. Comparison of the extent of granules is shown below.

(a)

(b)

(c)