Comparison of the Donor Age-Dependent and In Vitro Culture-Dependent Mesenchymal Stem Cell Aging in Rat Model

Figure 3

(a) Adipogenic differentiation of ASCs from 6 mth donors, p. 3; 2 yrs. donors, p. 3; 6 mth donors, p. 30; 2 yrs. donors, p. 30 and comparison of adipogenesis-specific gene expressions of CEBPA, ADIPOQ, and PPARγ in adipogenic-stimulated ASCs from young (6 mth) and aged rats (2 yrs.) cultured for 3 or 30 passages; (b) osteogenic differentiation of ASCs from 6 mth donors, p. 3; 2 yrs. donors, p. 3; 6 mth donors, p. 30; 2 yrs. donors, p. 30 and comparison of osteogenesis-specific gene expressions of ALPL, RUNX2, and IBSP in adipogenic-stimulated ASCs from young (6 mth) and aged rats (2 yrs.) cultured for 3 or 30 passages; (c) chondrogenic differentiation of ASCs from 6 mth donors, p. 3; 2 yrs. donors, p. 3; 6 mth donors, p. 30; 2 yrs. donors, p. 30 and comparison of chondrogenesis-specific gene expressions of COMP, SOX9, and ACAN in adipogenic-stimulated ASCs from young (6 mth) and aged rats (2 yrs.) cultured for 3 or 30 passages.

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