Eif2s3y Promotes the Proliferation of Spermatogonial Stem Cells by Activating ERK Signaling

Figure 1

The expression pattern of Eif2s3y in dairy goats. (a) Real-time PCR analysis of Eif2s3y expression levels in different tissues of adult dairy goats. All results were compared with the testis. (b) Real-time PCR analysis of Eif2s3y expression levels in the testes of dairy goats of different ages. All results were compared with that of 1-month-old goats. (c) Immunofluorescence staining of eIF2γ in the testes of 3-month-old dairy goats counterstained with Hoechst 33342. The white arrows indicated typical SSCs. Scale bars, 200 μm (up) and 100 μm (down). (d) Immunofluorescence staining of eIF2γ in the testes of 24-month-old dairy goats counterstained with Hoechst 33342. The white arrows indicated typical SSCs, and the red arrows indicated typical sperms. Scale bars, 200 μm (up) and 100 μm (down). Data are presented as and are represented by three independent repetitions; , , and .

(a)

(b)

(c)

(d)