Eif2s3y Promotes the Proliferation of Spermatogonial Stem Cells by Activating ERK Signaling

Figure 5

Eif2s3y promotes the colonization of goat SSCs in SSCT. (a) OeEif2s3y or Control SSCs were injected into the seminiferous tubules of busulfan-treated mice. The figure here shows two typical pictures of the testis during injection. Scale bars, 1 mm and 5 mm as indicated. (b) Morphology of testes and epididymides transfected with oeEif2s3y or Control SSCs. (c) The testicular (left) and epididymis (right) weight/body mass ratio in two groups. (d) H&E staining of the mouse testis 30 days after injection. Scale bars, 50 μm (left) and 20 μm (right). (e) Statistical plots of the diameter of seminiferous tubules (left) and the thickness of seminiferous epithelia (right) from oeEif2s3y and Control SSC-transplanted mice. Each group counted at least 30 round seminiferous tubules from 10 mice. (f) H&E staining of the mouse epididymis 30 days after injection. Scale bars, 50 μm (left) and 20 μm (right). (g) Statistical plots of the diameter of epididymis tubules from oeEif2s3y and Control SSC-transplanted mice. Each group counted at least 30 epididymis tubules from 10 mice. (h) RT-PCR analysis of the expression of Eif2s3y, Pcna, Zbtb16, and Cyclin D in Testis-Control and Testis-oeEif2s3y. (i) Immunofluorescence staining of DDX4 (green) in SSC-transplanted testes. The nuclei were stained with Hoechst 33342 (blue). Scale bar, 100 μm. These white arrows represented typical DDX4-positive germ cells. DDX4 is a representative marker for pan-germ cells. (j) Immunofluorescence staining of ZBTB16 (green) in SSC-transplanted testes. The nuclei were stained with Hoechst 33342 (blue). Scale bar, 100 μm. These white arrows represented typical ZBTB16-positive spermatogonial stem cells. ZBTB16 is a representative marker for undifferentiated spermatogonia. Data are presented as and are represented by three independent repetitions; , , and ; N.S. means .

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