Review Article

Electrical Stimulation Promotes Stem Cell Neural Differentiation in Tissue Engineering

Table 4

Pulsed current stimulation used in stem cell neural differentiation.

ES typeCell typeConductive materialStimulation parametersES effectReference

Pulsed currentESCs4-mm gap cuvette0, 5, 10, and 20 V, 5 pulses (950 ms interpulse interval)Increased differentiate into various types of neurons in vivoYamada M et al. [36]
Pulsed current electric fieldNSCs and MSCs1 cm long parallel electrodes20 and 40 kV/cm, 24 h, 503 ps, amplitude of 1016 V/m,Upregulation of NSCs astrocyte specific differentiationPetrella RA et al. [38]
Biphasic electrical stimulation (BES)Olfactory bulb NSCsFluorine-doped tin oxide glass plates25 mV/mm and 50 mV/mm, 8 ms pulses (20% duty cycle), 12 hImproving cell survival and preventing cell apoptosisWang L et al. [55]
BESFetal NSCsITO glasses electrodes100 Hz,4, 8, 16 and 32 mA/cm2 with 50 and 200 ms pulses, 4 or 7 daysPromote both the proliferation and neuronal differentiationChang KA et al. [56]
Pulsed electrical stimulationNeuro-spheresITO electrodes5 V, 30 HzEnhanced βIII-tubulin and calcium influxesTandon N et al. [57]
Pulsed currentHuman neural crest stem cellAu electrodes placed in a top bottom of 96 well plate2 or 20 Hz, 100 μs, 200 mV/mm, 24 hEnhanced nerve regeneration, increased Schwann cell differentiationDu J et al. [58]
Pulsed currentMouse NSCAg/AgCl electrodes300 mV/mm, 100 Hz, 50% duty cycle, 48 hInduced NSCs differentiation into neurons, astrocytes, and oligodendrocytes simultaneouslyChang HF et al. [59]
Pulsed currentNSCsPLGA/GO conductive composite membrane100 mV, 20, 100, and 500 Hz, 1 h/day, 3 daysPromote cell migration, adhesion and proliferation rates; promote neurite elongation and neuron differentiation, inhibited astrocytes differentiationFu C et al. [60]
Pulsed electric simulation a self-powered electrical simulation systemMSCReduced GO-PEDOT hybrid microfiber300 V, 30 μA, 21 daysIncreased βIII-tubulin and GFAP gene expressionGuo W et al. [61]