Adult Human Multipotent Neural Cells Could Be Distinguished from Other Cell Types by Proangiogenic Paracrine Effects via MCP-1 and GRO

Figure 3

Expression of MSC markers and in vitro differential potential into mesenchymal lineage cells of ahMNCs. (a) Protein levels of MSC markers were analyzed by flow cytometry. Number in each panel indicated percent of marker-positive cells. (b, c) Cells were maintained in osteogenic (b) or adipogenic (c) differentiation medium for 17 days (Diff). Undifferentiated cells (Undiff) were maintained in α-MEM supplemented with 5% FBS for 17 days. (b, c) Calcium deposit (b) and lipid vacuoles (c) were then stained with Alizarin red S and Oil red O solution, respectively. Scale bars, 100 μm.

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