Retinal Lineage Therapeutic Specific Effect of Human Orbital and Abdominal Adipose-Derived Mesenchymal Stem Cells

Figure 4

Differentiation potential of ABASCs to retinal precursors cells. (a, b) Coculture of p-RPE and ABASCs. (a) Prestaining of cells: ABASCs were marked with CSFE (green color), and RPE mature cells were marked with Violet Cell Trace (blue color). (b) FACS sorter: three distinct populations are defined after coculture: RPE, ABASCs, RPE and ABASCs which were fused together. (c) Fusion of cells as shown by one cell expressing both Violet Cell Trace (RPE tracer) and CFSE (OASC tracer). (d) Evidence of differentiation potential of ABASCs to retinal precursors cells by immunofluorescence. (A) Sorted ABASCs expressing cytoplasmic OTX2. (B) Sorted ABASCs in CFSE showing nuclear localized PAX6 expression. (C, D) Positive control of sorted RPE cells in Violet Cell Trace showing nuclear localized OTX2 expression and nuclear localized PAX6 expression (E, F) negative controls of undifferentiated ABSCs does not show any OTX2 expression and PAX6 expression. CT: cell trace. .

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