miR-129-5p Promotes Osteogenic Differentiation of BMSCs and Bone Regeneration via Repressing Dkk3

<div>miR-129-5p promoted osteoblast differentiation of BMSCs. BMSCs were transduced with a lentiviral vector containing miR-129-5p mimic, miR-129-5p inhibitor, or negative control (NC) lentivirus, respectively. 48 hours after transduction, cells were cultured in osteoblast induction medium. ALP staining was performed at days 7 and 14 following induction (a); corresponding ALP absorbance index was calculated (b). ARS staining of BMSCs was performed at days 14 and 21 following induction (c); corresponding ARS absorbance index was calculated (d). mRNA (e) and protein (f) levels of osteoblast-related genes Runx2, Bmp2, and OCN were detected by quantitative RT-PCR and western blot, respectively. <i>β</i>-Actin was used as an internal control. 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Stem Cells International

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Figure 3

Figure 3: miR-129-5p Promotes Osteogenic Differentiation of BMSCs and Bone Regeneration via Repressing Dkk3 