miR-129-5p Promotes Osteogenic Differentiation of BMSCs and Bone Regeneration via Repressing Dkk3

Figure 5

Knockdown of miR-129-5p suppressed bone regeneration. BMSCs transduced with lentivirus containing miR-129-5p inhibitor or control miRNA (NC) were loaded on matrigel scaffolds, respectively, and then implanted into the defect of murine calvarium. Mice were sacrificed at week 4 and week 8 after implantation, and then, skull samples were collected for analyses. (a) Image with three-dimensional reconstruction (coronal and sagittal sections) from Micro-CT revealed different regeneration effects in the miR-129-5p inhibitor group and the NC group. Quantitative analysis of the 3D images, including new bone volume relative to tissue volume (BV/TV) (b) and the bone mineral density (BMD) (c), was displayed. (d) Representative image of H&E staining (red arrow represented bone-like structures). (e) Immunohistochemical assessment of Dkk3, Runx2, and OCN in the miR-129-5p inhibitor group and the NC group (red arrow represented the positive cells). and .

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