miR-129-5p Promotes Osteogenic Differentiation of BMSCs and Bone Regeneration via Repressing Dkk3

<div>miR-129-5p activated the Wnt/<i>β</i>-catenin pathway and its downstream transcription factors in BMSCs. The mRNA (a) and protein (b) expression of <i>β</i>-catenin, Gsk-3<i>β</i>, and c-Myc were determined by quantitative RT-PCR and western blot in BMSCs transduced with a lentiviral vector containing miR-129-5p mimic, miR-129-5p inhibitor, or control (NC) lentivirus. (c) Quantitative analysis of protein expression levels of <i>β</i>-catenin, Gsk-3<i>β</i>, and c-Myc. (d) Immunofluorescence assays showing the expression and location of <i>β</i>-catenin in the indicated BMSCs. Scale bars, 50 <i>μ</i>m. All assays were repeated at least three times. 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Stem Cells International

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Figure 8

Figure 8: miR-129-5p Promotes Osteogenic Differentiation of BMSCs and Bone Regeneration via Repressing Dkk3 