Research Article

EZH2 Regulates Lipopolysaccharide-Induced Periodontal Ligament Stem Cell Proliferation and Osteogenesis through TLR4/MyD88/NF-κB Pathway

Figure 3

Targeting EZH2 prevented LPS-induced upregulation of TNFα, IL1β, and IL6. (a) The efficiency of shEZH2 was detected by western blot assay in PDLSCs. (b) Western blot showed the protein levels of H3K27me3 and EZH2 after LPS stimulation or LPS stimulation and shEZH2 in PDLSCs. (c) TNFα, IL1β, and IL-6 levels of PDLSCs were assessed by ELISA assays. Data are representative of three independent experiments. ; ; ns: not significant; EZH2: enhancer of zeste 2 polycomb repressive complex 2 subunit; LPS: lipopolysaccharide; shNC: sh-normal control; TNFα: tumor necrosis factor α; IL1β: interleukin 1 beta; IL6: interleukin 6.