Research Article

EZH2 Regulates Lipopolysaccharide-Induced Periodontal Ligament Stem Cell Proliferation and Osteogenesis through TLR4/MyD88/NF-κB Pathway

Figure 5

EZH2 knockdown inhibits TLR4/MyD88/NF-κB signaling to facilitate PDLSC osteogenesis. (a) Western blot showed the phosphorylated p65, phosphorylated IKB, TLR4, and MyD88 protein level transfected with the indicated plasmids in PDLSCs. (b) IF showed the distribution of p65 in the indicated treatment. (c, d) ALP activity in PDLSCs after the indicated treatments. (e) Western blot showed the Runx2 and OCN protein level after the indicated treatment in PDLSCs. (f) qPCR showed the mRNA level of Runx2 and OCN. (g, h) Alizarin red staining showed the role of EZH2 and LPS on the mineralization in PDLSCs. Data are representative of three independent experiments. ; ; ns: not significant; LPS: lipopolysaccharide; shNC: sh-normal control; p-p65: phosphorylated p65; p-IKB: phosphorylated IKB; TLR4: toll-like receptor 4; MyD88: myeloid differentiation primary response gene 88; PMA: phorbol ester; ALP: alkaline phosphatase assay; OCN: bone gamma-carboxyglutamate protein; Runx2: RUNX family transcription factor 2.