Functional Analysis of Ectodysplasin-A Mutations in X-Linked Nonsyndromic Hypodontia and Possible Involvement of X-Chromosome Inactivation

Figure 4

Effects of EDA on odontogenic differentiation of hDPSCs. (a, b) mRNA expression level of EDA and DSPP in hDPSCs. There were significant differences in the mRNA levels of wild-type and mutant EDA. (c) Western blot analysis of EDA and DSPP expression. (d) Human DPSCs transfected with mutant EDA expressed lower levels of p-p65 and p-IkBα than hDPSCs transfected with wild-type EDA. (e–h) Relative intensity of DSPP, EDA, p-p65, and p-IkBα. (i) Calcium nodule deposition of hDPSCs after transfection was examined by Alizarin Red S staining. Mutant EDA showed a decreased capacity of odontogenic differentiation compared with those transfected with wild-type EDA. Data are expressed as the . Each experiment was repeated three times with samples per group. and .

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