lncRNA-KCNQ1OT1: A Potential Target in Exosomes Derived from Adipose-Derived Stem Cells for the Treatment of Osteoporosis

Figure 3

Characteristics of ADSCs, primary osteoblasts, and ADSCs-Exos. (a) (1) ADSCs isolated from C57/BL6 mice had a typical fibroblastic-like morphology. Alizarin Red (2), Oil Red O (3), and Alcian Blue stainings (4) were positive after the induced osteogenic, adipogenic, and chondrogenic differentiations of ADSCs. The isolated ADSCs were positive for CD90 (5) while negative for CD34 (6). (b) (1) Primary osteoblasts isolated from C57/BL6 mice had shuttle, cone, or cube morphology. Alkaline phosphatase staining (2) and Alizarin Red S staining (3) were positive in the isolated osteoblasts. (c) ADSCs-Exos were positive for the exosomes’ markers, including CD9, CD81, CD63, and Alix; NAT and TEM results showed that the isolated exosomes were in complete form with the size of  nm. (d) Primary osteoblasts could uptake the PKH26-labeled ADSCs-Exos. Red: PKH26-labeled ADSCs-Exos. Blue: nuclei.

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