lncRNA-KCNQ1OT1: A Potential Target in Exosomes Derived from Adipose-Derived Stem Cells for the Treatment of Osteoporosis

Figure 7

KCNQ1OT1-Exos inhibit the effect of TNF-α in primary osteoblasts by sponging miR-141-5p. (a) Primary osteoblasts were treated with TNF-α and then treated with miR-NC, miR-141-5p, miR-141-5p+ADSCs-Exos, miR-141-5p+LV-NC-Exos, or miR-141-5p+LV-KCNQ1OT1-Exos, respectively. (b) The inhibitory effect of TNF-α on cell viability was blocked by miR-141-5p, and the treatment of ADSCs-Exos or LV-KCNQ1OT1-Exos partly reversed this phenomenon; ADSCs-Exos exerted a weaker stimulative effect on cell viability compared to LV-KCNQ1OT1-Exos; the expression of cleaved caspase-3 and Bax was enhanced by miR-141-5p but was attenuated following coculture with ADSC-Exos or LV-KCNQ1OT1-Exos. (c) As flow cytometry showed, miR-141-5p promoted cell apoptosis while the treatment of ADSC-Exos or LV-KCNQ1OT1-Exos inhibited that; ADSCs-Exos exerted a weaker inhibitory effect on cell apoptosis compared to LV-KCNQ1OT1-Exos. , , and .

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