Melatonin Alleviates LPS-Induced Pyroptotic Cell Death in Human Stem Cell-Derived Cardiomyocytes by Activating Autophagy

<div>Melatonin promotes autophagy in LPS-exposed hiPSC-CMs. (a) Transmission electron microscope images of hiPSC-CMs in LPS+MEL (1 <i>μ</i>mol/l) and LPS+MEL (10 <i>μ</i>mol/l) cells. M indicates representative mitochondria. Arrows indicate autophagosomes. Scale bar: 500 nm. (b) Representative immunofluorescent images for LAMP1 (green), LC3-II (red), <i>α</i>-actinin (purple), and DAPI (blue). Scale bar: 20 <i>μ</i>m. (c) Representative immunoblots of LC3-II, p62, and LAMP1. (d) Quantitative analysis of LC3-II. (e) Quantitative analysis of p62. 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Stem Cells International

fig5

Figure 5

Figure 5: Melatonin Alleviates LPS-Induced Pyroptotic Cell Death in Human Stem Cell-Derived Cardiomyocytes by Activating Autophagy 