Hyperosmotic Stress Induces a Specific Pattern for Stress Granule Formation in Human-Induced Pluripotent Stem Cells

Figure 1

SG assembly in hiPSCs/IMR90-1 and SH-SY5Y under hyperosmolarity stress. (a) Representative fluorescence microscopy images showing nontreated hiPSCs/IMR90-1 and SH-SY5Y cells treated with 50, 100, 200, 300, and 400 mM of sodium chloride stained with the robust SG marker (G3BP (green)). LIN28 is an RNA-binding protein involved in promoting pluripotency. Nucleus is stained in blue (Hoechst). Insets show magnified views of SGs. White arrows indicate SGs. Scale bar indicates 20 μm. (b) Percentage of hiPSCs/IMR90-1 with G3BP-positive SGs after 1 h treatment with the indicated concentrations of sodium chloride (50, 100, 200, 300, and 400 mM). The average percentage of cells with SGs is shown. Error bars indicate the ±standard deviation from 3 independent experiments. Approximately, 1000 cells were counted (distributed over 20 different fields within each coverslip).

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