Hyperosmotic Stress Induces a Specific Pattern for Stress Granule Formation in Human-Induced Pluripotent Stem Cells

Figure 3

The flow cytometric (FACS) analysis diagram of NaCl for hiPSCs/IMR90-1 and SH-SY5Y. (a) Representative flow cytometric multicolor gating of cells used to analyze the number of live/dead cells and representative profiles of FACS analysis after 1-hour treatment with NaCl. Cells were collected after 1-hour treatment with different concentrations of NaCl, stained with Annexin V and PI, and analyzed by FACS. Cells without NaCl treatment were used as negative controls ( in each group). The diagram can be divided into four regions that are defined as follows: the percentage of necrotic cells (Q1; PI/FITC +/−), the percentage of late apoptotic cells (Q2; PI/FITC +/+), the percentage of viable cells (Q3; PI/FITC −/−), and the percentage of early apoptotic cells (Q4; PI/FITC −/+). (b) Percentages of viable, early, and late apoptosis are presented in the graphs for both types of cells. Results represent four independent experiments with similar results.

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