Hyperosmotic Stress Induces a Specific Pattern for Stress Granule Formation in Human-Induced Pluripotent Stem Cells

Figure 7

The summary figure of the study. This summarizes a cellular mechanism that controls the assembly and disassembly of SGs induced by hyperosmotic stress in hiPSCs/IMR90-1. Upon gradient concentrations of hyperosmolarity treatment, the effect of increased cell osmolarity differs from one type of cell to another. Under 200 mM of NaCl, hiPSCs/IMR90-1 and SH-SY5Y showed SG formation. However, with a higher concentration, 400 mM, SGs disappeared in hiPSCs/IMR90-1. Reduced expression of tubulin may protect cells against hyperosmolarity stress while inhibiting SG formation without affecting stem cell self-renewal and pluripotency. Possible implications of microtubule organization, dynamic structural cellular components, on the response to hypertonic stress in hiPSCs were found.