Research Article
RAB37 Promotes Adipogenic Differentiation of hADSCs via the TIMP1/CD63/Integrin Signaling Pathway
Figure 3
RAB37 promotes secretion of TIMP1: (a) schematic of the screening strategy for upregulated cytokines in RAB37-mediated adipogenic differentiation of hADSCs; (b) fold changes of upregulated cytokines; (c) ELISA was performed to evaluate the concentration of TIMP1 in the supernatant of hADSCs during adipogenic differentiation (); (d) relative protein amount of RAB37 was determined by western blotting, ; (e) correlation analysis between the relative protein amount of RAB37 and the concentration of TIMP1 calculated using Pearson analysis; (f) proximity ligation assay (PLA) was used to examine the interaction between RAB37 and TIMP1 on day 5 of adipogenic differentiation. . Eight random views were counted, .
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