Design and Optimization of the Circulatory Cell-Driven Drug Delivery Platform

Table 2

Cargo loading conditions of encapsulation and backpack approaches.

Refs.	Loading method	Culture time	Incubation temperature	Drugs/NPs	Drug/NP size (nm)	Drug concentration for loading	Cell carrier type	Final loading amounts (μg drug per million cells)	Cancer type	In vitro/in vivo
[70]	Encaps	5 min	Unknown	Dox/TargetMAG NPs	50	2 μg/mL	T cell	Dox 0.192	N/A	In vitro
[18]	Encaps.	2 h	37°C	Dox/silica NPs	28.4	20 μg/mL	MA	Dox 16.6	Glioma	In vivo
[72]	Encaps.	4 h	37°C	Dox/poly(AAc-co-DSA) NPs	260	13.6 μg/mL	MO	Dox 1.44	Prostate cancer	In vivo
[67]	Encaps.	6 h	Unknown	Dox/liposome NPs	145	25 μg/mL	MA	Dox 4.4	Breast cancer	In vivo
[69]	Encaps.	8 h	37°C	PTX/PLGA NPs	135	8 ng/mL	MSC	PTX 1	Glioma	In vivo
[38]	Encaps.	12 h	Unknown	PTX/albumin NPs	100-130	200 μL PTX	NE	PTX 18	Gastric cancer	In vivo
[53]	BP	20 min	Unknown	Curcumin/chitosan NP-biotin	377	50 μg/mL	MSC	Curcumin 54.73	Lung cancer	In vivo
[59]	BP	30 min	37°C	NSC-87877/liposome NP-PEG	200		T cell	100 NPs/cell	Prostate tumor	In vivo
[12]	BP	30 min	4°C	SN-38/liposome NPs	340	Unknown	T cell	SN-38 0.4	Lymphoma	In vivo
[46]	BP	30 min	RT	Chlorin e6	Molecule	3 μg/mL	RBC	Ce6 molecules on the surface	Breast cancer	In vitro
[51]	BP	1 h	37°C	IL-15Sa/nanogel-PEG	121	0.67 μg/mL	T cell	IL-15Sa 7.68	Melanoma	In vivo
[52]	BP	1 h	37°C	Dox/SN-anti-CD90/CD73	152.9	100 μg/mL	MSC	1500 NPs/cell	Glioma	In vivo
[24]	BP	1 h	4°C	ZnF16Pc/biotin-ferritin	15-18	Unknown	RBC	ZnF16Pc molecules on the surface	Glioma	In vivo

Encaps. = encapsulation; BP = backpack; RT = room temperature. For other abbreviations, please refer to the abbreviation list.