PTHG2 Reduces Bone Loss in Ovariectomized Mice by Directing Bone Marrow Mesenchymal Stem Cell Fate

Figure 4

PTHG2 promotes osteogenic differentiation of BMSCs and inhibits adipogenic differentiation. (a) BMSCs were cultured in osteoblast medium for 7 days, and the ALP positive area was measured. (b) The relative expression of osteoblast marker gene (OCN/Osterix/RUNX2) following PTHG2 treatment was quantified by real-time PCR. (c) Western blotting assays of ALP and RUNX2 during osteogenic differentiation. (d) Oil red O staining detected the effects of PTHG2 on BMSCs adipogenic differentiation and quantity. (e) The relative expression of adipocyte marker gene (Fabp4/PPARɣ/Adiponectin) following PTHG2 treatment was quantified by real-time PCR. (f) Western blotting assays of PPARɣ and adiponectin during adipogenic differentiation. (); , , , .

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