3D Electrospun Nanofiber-Based Scaffolds: From Preparations and Properties to Tissue Regeneration Applications

Figure 9

Nanofiber yarn/hydrogel composite 3D scaffolds with alignment, conductivity, and anisotropy for cardiac tissue engineering. Schematic illustration of (a) PCL/SF/CNT nanofiber yarns prepared by wet-dry electrospinning and (b) NFYs-NET scaffolds fabricated by a weaving technique. (c) 3D composite scaffold fabricated via encapsulating two layers of NFYs-NET with orthogonal orientation within UV crosslinked GelMA hydrogel (i). F-Actin (green) staining of CMs cultured on the NFYs-NET layer with horizontal direction (iii) and vertical direction (iv) within a 2-layer 3D scaffold. (d) Coculture of CMs and ECs within yarn/hydrogel 3D composite scaffolds. CMs were cultured on the NFYs-NET layer while ECs with green fluorescent protein were encapsulated within a hydrogel shell. Reproduced with permission from [67]. Copyright © 2017, American Chemical Society.

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