Reestablishment of Redox Homeostasis in the Nociceptive Primary Afferent as a Mechanism of Antinociception Promoted by Mesenchymal Stem/Stromal Cells in Oxaliplatin-Induced Chronic Peripheral Neuropathy

Figure 3

Effect of MSC on mitochondrial morphology and morphometry of dorsal root ganglia neurons of mice with OXL-induced chronic neuropathy. Electron micrographs representing dorsal root ganglia (DRG) cross-sections from nonneuropathic mice (A, D) (control group) and neuropathic mice treated with vehicle (B, E) (OXL+vehicle) or with MSC (C, F) (OXL+MSC). Analyses were performed 4 weeks after treatments. (A) Electron micrograph of the control group with numerous typical mitochondria. (D) Higher magnification (50,000x) of the highlighted area in (A), showing typical mitochondria, with intact membrane and mitochondria cristae (arrow). (B) Neuropathic mice treated with vehicle presented numerous atypical mitochondria. (E) Higher magnification image showing atypical mitochondria, characterized by increased organelle area (arrowhead) and accumulation of electron-dense amorphous material on mitochondrial poles (cross). MSC-treated neuropathic mice (C, F) presented fewer mitochondrial atypia relative to vehicle-treated neuropathic mice. (A–C) (10,000x magnification) and 0.5 μm (D–F) (50,000x magnification). (G) shows the percentage of atypical mitochondria found in DRG. Data are expressed as ; mice per group. Statistical significance relative to the control group (); ^#statistical significance relative to the OXL+vehicle group (), as determined by one-way ANOVA followed by Tukey’s multiple comparison test.

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