Adventitial Progenitor Cells of Human Great Saphenous Vein Enhance the Resolution of Venous Thrombosis via Neovascularization

Figure 7

CD34⁺CD117⁺HGSV-AdPCs differentiated into endothelial cells in vitro. CD34⁺CD117⁺HGSV-AdPCs were isolated with FACS and cultured in the presence of VEGF for 7 days. (a) The mRNA expression of CD31, VEGFR2, and vWF. (b) The result of Western blotting of CD31 and VEGFR2 proteins in the induction and control groups. (c) The differentiation result of immunofluorescent staining with antibodies CD31 and VEGFR2 in control and induction groups. (d) The tube formation observed under inverse microscope at 6 hours culture in control and induction groups. (e) The difference of tube formation at 6 hours culture between control and induction groups. Scale bars: 10 μm (c); 200 μm (d). Magnification: 200x (a, d).

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