NAT10 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Mediating N4-Acetylcytidine Modification of Gremlin 1

Figure 2

NAT10 positively regulated the osteogenic capacity of MSCs. (a) Sh-NAT10 significantly decreased the protein level of NAT10. (b) ARS staining and quantification were decreased in the Sh-NAT10 group compared to the negative control (NC) lentivirus group. . (c) ALP staining and activity were decreased in the Sh-NAT10 group compared to the NC lentivirus group. . (d) RUNX2, OPN, and OCN mRNA expression was decreased in the Sh-NAT10 group. (e) Over-NAT10 significantly increased the protein level of NAT10. (f) ARS staining and quantification were increased in the Over-NAT10 group compared to the NC lentivirus group. . (g) ALP staining and activity were increased in the Over-NAT10 group compared to the NC lentivirus group. . (h) RUNX2, OPN, and OCN mRNA expression was increased in the Over-NAT10 group. indicates . All experiments were repeated three times. The induction group indicates MSCs undergoing osteogenic differentiation without other treatment. NC1 indicates control lentivirus-transfected MSCs undergoing osteogenic differentiation.

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