Primary Cilia as a Biomarker in Mesenchymal Stem Cells Senescence: Influencing Osteoblastic Differentiation Potency Associated with Hedgehog Signaling Regulation

Figure 5

Depletion of primary cilia with a siRNA targeting IFT88 suppressed cilia formation and the gene expression of Gli1 and ALP. (a) Representative images showing the loss of primary cilia in P0 MSCs incubated with IFT88 siRNA. (b) The resultant decrease in ciliation was confirmed. (c) The gene transcriptional activities of Gli1 and ALP were compared, and it was found that loss of primary cilia suppressed hedgehog signaling and osteogenic differentiation. , Student’s -test, versus MSCs treated with negative control siRNA.

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