Ligustrum japonicum Thunb. Fruits Exert Antiosteoporotic Properties in Bone Marrow-Derived Mesenchymal Stromal Cells via Regulation of Adipocyte and Osteoblast Differentiation

Figure 10

Effect of salidroside (10 μM) on the levels of (a) p38, ERK, and JNK MAPKs and (b) AMPK. Phosphorylated (p-) and total protein levels analyzed by Western blotting using adipo-induced hBM-MSC lysates at day 14 of differentiation. (c) Time-dependent p-AMPK levels were obtained at the designated times from adipo-induced compound C-treated hBM-MSCs lysates with or without salidroside (10 μM). Compound C was used as an inhibitor of AMPK phosphorylation. Western blot bands were densitometrically quantified and plotted as relative percentage of the untreated differentiated control group. Phosphorylation levels were measured via normalization of the phosphorylated (p-) protein against their total protein levels. β-Actin was used as internal loading control. and vs. the untreated differentiated control group; ^# and ^## vs. the differentiated control group without compound C and salidroside treatment.

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