Inhibition of Notch Signaling Promotes the Differentiation of Epicardial Progenitor Cells into Adipocytes

Figure 1

EAT located at the left and right atrial–ventricular groove in postnatal 8-week-old mouse hearts. (a, b) Oil red O staining showed that EAT located at the atrial–ventricular groove in mouse. The boxed regions were shown at higher magnification in A’ and B’. (c, d) H&E staining showed that EAT was present in the atrial–ventricular groove underneath the epicardium. The boxed regions were shown at higher magnification in C’ and D’. (e, f) Immunofluorescence staining for adipocyte marker perilipin-1 showed the epicardial adipocytes were present in the right and left atrial–ventricular groove. The boxed regions were shown at higher magnification in E’ and F’. (g–l) The genetic lineage tracing methods were used to trace the EPCs. Immunofluorescence staining for adipocyte markers perilipin-1 (red) and FABP4 (red) was partly colocalized with the lineage marker EYFP (green) in the same section.