Inhibition of Notch Signaling Promotes the Differentiation of Epicardial Progenitor Cells into Adipocytes

Figure 3

Notch signaling and its target genes in EPCs were regulated by the treatment of jagged-1 and DAPT for 2 days. (a, b) The mRNA expression of Notch signaling and its target genes were significantly upregulated with the treatment of jagged-1 for 2 days compared with the control. (c, d) The mRNA expression of Notch signaling and its target genes were significantly downregulated with the treatment of DAPT for 2 days compared to the control. The values were calculated by -test. , , and for pairwise comparison. (e) Immunofluorescence staining showed that activated Notch-1 was significantly upregulated with jagged-1 treatment while decreased with DAPT treatment. (f) Immunofluorescence staining showed that Hes-1 was significantly upregulated with jagged-1 treatment while decreased with DAPT treatment. The values were calculated by ANOVA. , , and for pairwise comparison among the groups.