Different Neurogenic Potential in the Subnuclei of the Postnatal Rat Cochlear Nucleus

Figure 2

Neurospheres from all CN subnuclei contain neural progenitor cells. These spheres adhered to glass coverslips, coated with poly-d-lysine and laminin-1. After 48 h in NSC medium, these showed centrifugally outgrowing branches and emigrating cells, stained for β-tubulin. Cells in the neurospheres of all subnuclei showed a positive staining for the transcription factors Atoh-1 (a–c) and Sox-2 (d–f). (g–i) Neural stem cells were identified by the markers DCX (doublecortin) and nestin (j–l). Cell nuclei were stained with DAPI.