Different Neurogenic Potential in the Subnuclei of the Postnatal Rat Cochlear Nucleus

Figure 5

Neurogenesis in the CN subnuclei. Preparations from PND 9 animals were incubated as whole-mount organ cultures for 48 h in stem cell medium and the S phase marker BrdU. The immunohistological analysis showed the expression of neural stem cell markers and mitotic activity of individual cells. The transcription factors Atoh-1 and Sox-2 as well as the neural stem cell markers DCX and nestin were detected in preparations from all subnuclei. All whole-mount organ cultures showed a BrdU uptake of individual cells after an incubation period of 48 h. Cell nuclei were stained with DAPI.