Different Neurogenic Potential in the Subnuclei of the Postnatal Rat Cochlear Nucleus

Figure 7

Representative illustration of the flow cytometry analysis with propidium iodide and anti-phospho-histone-H3 from AVCN, PVCN, and DCN NSCs. (a–c) Population of vital cells (P1) in the plot according to width by the area of propidium iodide’s fluorescence intensity. P2 shows the population with double DNA content of the G2 and M phases. (d–f) Plot of the population of viable cells gated according to propidium iodide (width) by phospho-histone-H3 (height). P3 shows the population of viable cells with double DNA content—simultaneously stained positively with anti-phospho-histone-H3. (g–i) Histogram of the propidium iodide fluorescence (height) in the viable cell population for quantifying the cell cycle: P4 corresponds to the G0/G1 phase, P5 corresponds to the S phase, and P6 corresponds to the G2 /M phase.