Stem Cells International / 2021 / Article / Fig 3

Research Article

Chaetocin Promotes Osteogenic Differentiation via Modulating Wnt/Beta-Catenin Signaling in Mesenchymal Stem Cells

Figure 3

Treatment of Chaetocin increase Wnt/β-catenin activities. (a) Western blot shows nuclear β-catenin protein levels were elevated by Chaetocin treatment in MSC culture at day 7. Histone H1 was used as a loading control. (b) Real-time RT-PCR analysis shows that Wnt/β-catenin target genes (Cnnd1, Axin2, Myc, and Dkk1) were significantly increased by treatment with Chaetocin. PCR data is expressed as of three independent experiments (). (c) WB results show that Cnnd1, Axin2, and Myc were significantly increased after Chaetocin treatment.

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