Intestinal Organoids in Colitis Research: Focusing on Variability and Cryopreservation

Figure 1

ZellShield® does not affect organoid kinetics. Comparison of the gene expression levels in colonoids over the course of 12 hours after the administration of fresh medium with (+ ZS) and without (Ø ZS) ZellShield® (each: ). The graphs show the means with SDs. , , , and . (a) Relative Ocln expression, ANOVA (; ). (b) Relative Zo-1 expression, ANOVA (; ). (c) Relative Cldn4 expression, ANOVA (; ). (d) Relative Tnfα expression, ANOVA (; ). (e) Relative Ki67 expression, ANOVA (; ).

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