Intestinal Organoids in Colitis Research: Focusing on Variability and Cryopreservation

Figure 2

Experimental data are predominantly affected by interexperimental differences. The technical and experimental reproducibility and the biological variability in colonoids were compared using the EcN infection model. , , , and . (a) Technical replicates. The graphs plot individual values of five representative organoid lines with the technical replicate mean and SD values; the outliers, as identified using the Grubbs outlier test, are shown as black dots. (b) Experimental and biological replicates. The graphs plot five independent experiments with the technical replicate means from three different organoid lines (biological replicates) per experiment plus the overall mean and SD per experiment; the outliers, as identified using the Grubbs outlier test, are shown as red triangles; ANOVA: Ocln (, ), Zo-1 (, ), Cldn4 (, ), Tnfα (, ), and Ki67 (, ). (c) Pooled data. The graphs plot the technical replicate means of all biological replicates () from all independent experiments plus the overall mean and 95% CI.

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