Intestinal Organoids in Colitis Research: Focusing on Variability and Cryopreservation

Figure 3

Characterization of freshly isolated and cryopreserved colonoids during growth and after EcN infection. (a) Representative light phase-contrast images. (b) Immunofluorescent staining for epithelial cell adhesion molecule (CD326), KI67, and tight junction protein 1 (ZO-1). (c) The gene expression levels in freshly isolated colonoids () and cryopreserved and thawed organoids () of the same passage were compared before and after infection with EcN. The graphs plot the pooled technical replicate means of biological replicates plus the overall mean and 95% CI. , , , and . Relative Slc5a1 expression (ANOVA: ; ). Relative Chga1 expression (ANOVA: ; ). Relative Muc2 expression (ANOVA: ; ). Relative Ki67 expression (ANOVA: ; ).

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