Intestinal Organoids in Colitis Research: Focusing on Variability and Cryopreservation

Figure 5

Gene set enrichment analysis. Significantly (adjusted value < 0.01) upregulated genes in EcN compared to control samples originating from fresh and cryopreserved colonoids, respectively, were examined for their similarity to gene sets of the KEGG 2019 Mouse library via Enrichr gene set enrichment analysis web server. (a) Top enriched terms in the KEGG 2019 Mouse library, with P values. Asterisk symbolizes the term has an adjusted value < 0.05. (b) Each hexagon represents one gene set from the KEGG 2019 Mouse library. The brighter the blue color is, the more similar and, therefore, significant the specific gene set. Hexagons that are grouped together represent similar gene sets.

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