Proliferin-1 Ameliorates Cardiotoxin-Related Skeletal Muscle Repair in Mice

Figure 4

rPLF-1 alleviated the expressions of desmin and laminin proteins in the gastrocnemius muscles at the indicated days after CTX injection. (a, b) Double immunofluorescence was performed with the mouse mAb against desmin (red) and the rabbit polyclonal antibody (pAb) against laminin-5 (green). Representative images and quantitative data of the desmin protein expression in the gastrocnemius muscle of both experimental groups. (c) Double immunofluorescence was performed with goat pAb against integrin-α7 (red) and rabbit mAb against CD34 (green). Representative images and quantitative data show the numbers of CD34⁺/integrin-α7⁺ muscle stem cells (MuSCs). (d) Immunostaining was performed with mouse mAb against mouse monoclonal proliferating cell nuclear antigen (PCNA). Representative images and quantitative data show PCNA-positive cells. Red arrowheads: CD34⁺/integrin-α7⁺ cells or PCNA⁺ cells. Data are (–7). and vs. controls by Student’s -test.

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