Proliferin-1 Ameliorates Cardiotoxin-Related Skeletal Muscle Repair in Mice

Figure 9

PLF-1 blocking accelerated the impaired desmin and laminin expressions in the gastrocnemius muscles postinjury. (a, b) Double immunofluorescence was performed with the mouse monoclonal antibody (mAb) against desmin (red) and the rabbit polyclonal antibody (pAb) against laminin-5 (green). Representative images and quantitative data show the contents of desmin proteins in the gastrocnemius of both experimental groups. (c) Double immunofluorescence was performed with goat pAb against integrin-α7 (red) and rabbit mAb against CD34 (green). Representative images and quantitative data show the numbers of CD34⁺/integrin-α7⁺ muscle stem cells (MuSCs) in the gastrocnemius muscles of both experimental groups. (d) Immunostaining was performed with mouse mAb against proliferating cell nuclear antigen (PCNA). Representative images and quantitative data show PCNA-positive cells in the gastrocnemius muscles of both groups. Red arrowheads: CD34⁺/integrin-α7⁺ cells or PCNA⁺. Data are (–8). and vs. controls by Student’s -test.

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