An In Vitro and In Vivo Comparison of Osteogenic Differentiation of Human Mesenchymal Stromal/Stem Cells

Table 2

Summary of different osteogenic differentiation media reported in the literature.

Reference	Cell density	Assays/stains	Passage	Induction time (days)	Basal culture medium	FBS	Antibiotics	Dexamethasone (μM)	Ascorbate-2-phosphate (μM)	B-glycero-phosphate (mM)
Cai et al. 2014 [162]	NI	Alizarin Red S and ALP	2	21	DMEM-low glucose (lg)	10%	100 units/mL	0.01	155.26
Vieira et al. 2010 [163]	NI	Von Kossa	3	21	NI	10%	NI	0.1	50
Nishimura et al. 2015 [129]			5	14	DMEM	10%	100 units/mL	0.05	0.0002	10
Bieback et al. 2004 [164]		Von Kossa	NI	21	Cell systems	10%	NI	0.1	50	10
Waterman et al. 2010 [165]	(6-well)	Alizarin Red S	NI	NI	NI	NI	NI	0.1	50
Elashrya et al. 2019 [166]	(6-well)	Alizarin Red S	2-3	NI	DMEM	10%	100 U/mL	0.1	60	10
Li et al. 2015 [167]	5 000 cm^2	ALP	NI	NI	DMEM-high glucose	10%	100 U/mL	0.01	155.26	10
Sotiropoulou et al. 2006 [168]	NI	Von Kossa	NI	NI	DMEM-lg	10%	50 μg/mL Gentamicin	1	50	10
Rada et al. 2011 [169]	NI	Alizarin Red S	NI	21	α-MEM	10%	1%	0.1	155.26	10
Meuleman et al. 2006 [170]	NI	Von Kossa	1	14	α-MEM	NI	NI	0.1	60	10
Sasaki et al. 2008 [171]	NI	Von Kossa	NI	NI	DMEM	10%	0.1 μM	-	50	10
Zuk et al. 2001 [172]	NI	ALP or Von Kossa	1	14	DMEM	10%	1%	1	50	10
Bunnell et al. 2008 [173]	NI	Alizarin Red S	NI	14	α-MEM	20%	1%	0.001	50	2
Wagner et al. 2005 [111]		ALP or Von Kossa	NI	21	DMEM	10%	-	1	200	10

NI: not indicated; DMEM: Dulbecco’s modified Eagle’s medium; ALP: alkaline phosphatase.