Efficiently eliminated B lymphoma cells Ka539 in vivo and in vitro
53
Mouse inducible HoxB4 embryonic stem cells
EB formation and coculture with OP9 stromal cells
Recombinant KL, IL-6, IL-3, TPO, VEGF, Flt-3L, and M-CSF
MDSCs
CD115, Ly-6C
53.9%
Prevented GVHD following allogeneic bone marrow transplantation
61
Mouse-induced pluripotent stem cells
Coculture with OP9 stromal cells and hepatic stellate cells
GM-CSF
MDSCs
CD11b, CD80, CD86 and B7-H1
Upto 80% CD11b+CD11clow/−and 20% CD11b+CD11chigh
Regulated T cell responses in vitro and in vivo and diminished CD8+ T cell infiltration and ALT leakage in an autoimmune hepatitis murine model
65
Human-induced pluripotent stem cells Human embryonic stem cells
Coculture with OP9 stromal cells for hematopoietic differentiation and isolated CD235a/CD41a–CD45+ for myeloid progenitors generation
M-CSF, IL-1β
Macrophages
CD115, CD163, CD14, and CD68
35%~44%
The ability of a self-quenched conjugate of ovalbumin (DQ-OVA) uptake, processing, and allogeneic stimulation
69
Human-induced pluripotent stem cells
In earlier stage: coculture with OP9 stromal cells; in later stage: cultured in the presence of GM-CSF and M-CSF without OP9 cells
GM-CSF, M-CSF
Macrophages
CD11b, CD14 and CD68
Unmentioned
Ingested both immunoglobulin G-opsonized and unopsonized zymosan particles and showed chemotactic response to C5a. Inhibited the growth of BALL-1 cells in vivo and in vitro
BMP-4, bFGF, VEGF, SCF, IGF-1, IL-3, M-CSF, and GM-CSF
CAR-macrophages
CD11b, CD14, and CD163
~100% of CD11b and CD14 expression
Inhibited CD19-expressingK562 or leukemia cells mesothelin-expressing OVCAR3/ASPC1 ovarian/pancreatic cancer cells in vitro and ovarian cancer cells HO8910 in vivo
74
Human embryonic stem cells
Coculture with mouse bone marrow stromal cell line S17 and cocultured with AFT024 stromal cells
Ascorbic acid, 2-ME, IL-15, IL-3, IL-7, Flt-3L, and SCF
NK cells
CD56
About 30%~40% on day 28
Showed significant cytolytic activity to K562 cells in vitro and upregulate IFN-γ production in response to IL-12/IL-18 stimulation
86
Human embryonic stem cells and induced pluripotent stem cells
Spin EB formation
BMP-4, bFGF, VEGF, IL-15, IL-3, IL-7, Flt-3L, and SCF
NK cells
CD45, CD56
>90%
Killed against K562 cells and MOLM13 cells in vitro
87
Human embryonic stem cells and induced pluripotent stem cells
Completely chemically defined condition in two-dimensional culture
BMP-4, VEGF, IL-15, IL-7, Flt-3L, and SCF
NK cells
CD56
Killed K562 cells in vivo and in vitro
88
Human embryonic stem cells
Cocultured with C3H10T1/2 or OP-9 cells
IL-6, IL-11, SCF, TPO, and heparin
Platelets
CD41a, CD42b
Most small particles positive for CD41a and CD42b
Displayed integrin αIIbβ3 activation and spreading in response to ADP or thrombin
100
Human embryonic stem cells and induced pluripotent stem cells