Adipose-Derived Stem Cell-Derived Extracellular Vesicles Inhibit the Fibrosis of Fibrotic Buccal Mucosal Fibroblasts via the MicroRNA-375/FOXF1 Axis

Figure 6

EV-carried miR-375 inhibits the fibrosis of fBMFs via targeting FOXF1. EVs-miR-375 and plasmid overexpressing FOXF1 (pc-FOXF1)/pc-NC were transfected into fBMFs. (a, b) The successful transfection of FOXF1 was verified using RT-qPCR and WB. (c) CCK-8 assay was used to detect the proliferation of fBMFs. (d, e) Transwell assay was used to detect the migration and invasion of fBMFs. (f) Flow cytometry was used to detect the apoptosis of fBMFs cells. (g, h) RT-qPCR and WB were used to detect the levels of proapoptotic proteins (cleaved-caspase-3 and Bax), antiapoptotic protein Bcl-2, and fibrosis markers (α-SMA, collagen I, and collagen III). pc-NC/pc-FOXF1: fBMFs transfected with pc-NC/pc-FOXF1; EVs-miR-375+pc-NC/EVs-miR-375+pc-FOXF1: combined treatment of EVs-miR-375 and pc-NC/pc-FOXF1 in fBMFs. The experiment was repeated 3 times, and the data were expressed as . Comparison among groups was analyzed using one-way ANOVA, followed by Tukey’s multiple comparisons test. and .

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