Icariin Promotes Survival, Proliferation, and Differentiation of Neural Stem Cells In Vitro and in a Rat Model of Alzheimer’s Disease

Figure 1

Verifications on the locations of fimbria-fornix transection (FFT) surgery and NSC transplantation in vivo. (a) Flow chart of experimental schedule in vivo. (b) Unilateral FFT surgery was conducted on rats (red arrow indicates the locations of FFT surgery), and (c) Nissl staining was conducted to verify the unilateral FFT-induced brain injury. After FFT surgery, (d) NSCs () were injected into the basal forebrain of rats: AP: +0.6 mm, LL+0.6 mm, and DV-5.5 mm from the bregma (red arrow). 28 days later, (e) the BrdU immunostaining indicated the survived NSCs near the needle track in the medial septum (MS) of the basal forebrain. (f) Representative images of BrdU-labeled transplanted NSCs in the brain of FFT rats. Scale . (g) Quantitative analysis of the number of BrdU-positive cells in the NSC transplantation group and NSC transplantation-combined ICA treatment () group of FFT rats. Data are expressed as the , . , the vs the NSC transplantation group. LV: lateral ventricles.

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