Icariin Promotes Survival, Proliferation, and Differentiation of Neural Stem Cells In Vitro and in a Rat Model of Alzheimer’s Disease

Figure 3

ICA promoted the migration ability and differentiation of NSCs in vitro. (a) Representative images of individual NSCs migrated from the neurospheres incubated in B27 medium without bFGF and EGF at 1, 3, 7, and 14 days. Scale . (b) Quantitative analysis of the migration distance of the neurospheres at different time points after ICA incubation. Data are expressed as the , . (c) Immunostaining of Tuj1 or GFAP to determine the effect of ICA on the differentiation of NSCs after seven days of incubation. Scale . (d) Quantitative analysis of the percentage of Tuj1 or GFAP-positive cells to Hoechst-labeled NSCs. Data are expressed as the , . , drug-treated groups vs the control group.

(a)

(b)

(c)

(d)